5-AMINO-1MQ

$45.00

Small-Molecule NNMT Inhibitor · 5-Amino-1-Methylquinolinium Salt

5-Amino-1MQ is a membrane-permeable, small-molecule quinolinium derivative that functions as a selective, competitive inhibitor of nicotinamide N-methyltransferase (NNMT). First characterized by researchers at the University of Texas Medical Branch (UTMB) and University of Florida in 2017–2018, 5-Amino-1MQ binds within the NNMT active site, blocking the transfer of methyl groups from S-adenosylmethionine (SAM) to nicotinamide. By suppressing NNMT catalytic activity, 5-Amino-1MQ prevents the depletion of intracellular methyl donors and salvages nicotinamide for conversion back into the NAD⁺ salvage pathway. In preclinical rodent models of diet-induced obesity and metabolic dysfunction, NNMT inhibition via 5-Amino-1MQ has been shown to reduce white adipose tissue accumulation, increase intracellular NAD⁺ concentrations, upregulate SIRT1 activity, and enhance skeletal muscle metabolic energy expenditure.

Supplied at 5mg.

Description

Overview

5-Amino-1MQ (5-amino-1-methylquinolinium) is a cell-permeable small molecule that functions as a selective, competitive inhibitor of nicotinamide N-methyltransferase (NNMT). It is a non-peptidic compound engineered to block the enzymatic breakdown of nicotinamide.

In preclinical research, 5-Amino-1MQ binds within the active site of NNMT to inhibit the transfer of methyl groups from SAM to nicotinamide. This action increases intracellular NAD+ levels, restores methyl-donor pools, stimulates SIRT1 activity, and increases lipid oxidation and energy expenditure in adipocyte and skeletal muscle models.

Key Technical Specifications

Structure	`5-amino-1-methylquinolinium salt`
Molecular Formula	C₁₀H₁₁N₂⁺ (cation) / C₁₀H₁₁IN₂ (iodide salt)
Molecular Weight	159.21 g/mol (cation) / ~286.11 g/mol (iodide salt)
CAS Number	42464-96-0 (Iodide salt)
Solubility	Water / DMSO
Primary Class	Small-Molecule NNMT Inhibitor / Metabolic Modulator

Biochemical Mechanism & Pathway Dynamics

1. NNMT Active-Site Blockade

By competitively binding NNMT, 5-Amino-1MQ prevents the conversion of nicotinamide into 1-methylnicotinamide (MNA), preserving intracellular S-adenosylmethionine (SAM) levels for essential nuclear epigenetic methylations.

2. NAD+ Salvage & Sirtuin 1 Signaling

NAD+ Restoration: Rescued nicotinamide enters the NAMPT salvage pathway, causing intracellular accumulation of NAD+.
Metabolic Acceleration: Increased NAD+ activates SIRT1 deacetylase activity, stimulating mitochondrial respiration and reducing white adipose tissue mass in research models.

Preclinical Literature: Research by Neelakantan et al. (Biochemical Pharmacology, 2018) established that selective NNMT inhibition with 5-Amino-1MQ reduced adipocyte hypertrophy and reversed diet-induced obesity in mouse models without modifying calorie intake.

For laboratory research, educational, and analytical reference only. Strictly not for human or veterinary use.